Hotspot

petG-trnW-CCA

LSC · 65473-65588 · 116 bp

Back to Eulalia

Pi 0.0776
MarkerSeek score 79.10
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
116
variable_sites
9
pi
0.0776
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 GGGATTATTCGTGACTGCGT CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 213-2924 bp 1.0 79.2
petG-trnW-CCA_p2 AGCGGGATTATTCGTGACTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 216-2926 bp 1.0 77.9
petG-trnW-CCA_p3 GCGTATTTGCAATACAGGCG CAAAACCCGATGTCGTAGGT 50.0 50.0 58.3 57.9 197-2925 bp 1.0 77.7
petG-trnW-CCA_p4 GGGATTATTCGTGACTGCGT CCCGATGTCGTAGGTTCAAA 50.0 50.0 58.1 57.6 208-2925 bp 1.0 77.0
petG-trnW-CCA_p5 GGGATTATTCGTGACTGCGT TCCAAAACCCGATGTCGTAG 50.0 50.0 58.1 57.6 215-2925 bp 1.0 77.0