Hotspot

atpB-rbcL

LSC · 55727-56502 · 776 bp

Back to Eulalia

Pi 0.0184

MarkerSeek score 65.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 776
variable_sites: 14
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	958-2928 bp	1.0	78.3
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	957-2928 bp	1.0	78.3
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	955-2926 bp	1.0	78.1
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	954-2926 bp	1.0	78.0
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	57.7	58.2	973-2926 bp	1.0	77.8