Hotspot

trnT-UGU-trnL

LSC · 47896-48682 · 787 bp

Back to Eulalia

Pi 0.0146

MarkerSeek score 66.50

Primer available yes

Label trnT-UGU-trnL

Feature Metadata

label_name: trnT-UGU-trnL
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 11
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	888-2926 bp	1.0	81.6
trnT-UGU-trnL_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	880-2925 bp	1.0	81.3
trnT-UGU-trnL_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	858-2926 bp	1.0	80.8
trnT-UGU-trnL_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	878-2926 bp	1.0	79.7
trnT-UGU-trnL_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	887-2925 bp	1.0	79.5