Hotspot

trnT-GGU-trnE-UUC

LSC · 15949-16417 · 469 bp

Back to Eulalia

Pi 0.0527

MarkerSeek score 74.30

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 24
pi: 0.0527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AAAATGGAACTTCCGGGCTT	TTGAACTACAATCCCTGCGG	45.0	50.0	58.0	57.9	1597-2927 bp	1.0	82.2
trnT-GGU-trnE-UUC_p2	AAATGGAACTTCCGGGCTTT	TTGAACTACAATCCCTGCGG	45.0	50.0	58.0	57.9	1596-2927 bp	1.0	82.2
trnT-GGU-trnE-UUC_p3	AAAAATGGAACTTCCGGGCT	GCATCGACCCAGGAAGAATT	45.0	50.0	58.0	58.0	1149-2926 bp	1.0	82.1
trnT-GGU-trnE-UUC_p4	AAAATGGAACTTCCGGGCTT	GCATCGACCCAGGAAGAATT	45.0	50.0	58.0	58.0	1148-2926 bp	1.0	82.1
trnT-GGU-trnE-UUC_p5	AAATGGAACTTCCGGGCTTT	GCATCGACCCAGGAAGAATT	45.0	50.0	58.0	58.0	1147-2926 bp	1.0	82.1