Hotspot

trnG-UCC-trnM-CAU

LSC · 14066-15819 · 1754 bp

Back to Eulalia

Pi 0.0229
MarkerSeek score 65.60
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1754
variable_sites
39
pi
0.0229
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 1868-2925 bp 1.0 43.2
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1838-2925 bp 1.0 43.0
trnG-UCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT GACTTCTGTCTTACCAAGGCA 38.1 47.6 55.5 57.6 1896-2927 bp 1.0 42.7
trnG-UCC-trnM-CAU_p4 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1834-2927 bp 1.0 42.3
trnG-UCC-trnM-CAU_p5 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1835-2927 bp 1.0 42.3