Hotspot

ycf1

SSC · 128177-133783 · 5607 bp

Back to Eugenia

Pi 0.0306

MarkerSeek score 57.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5607
variable_sites: 254
pi: 0.0306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATCCTCTGCCACGTTTTCT	CTGTCGTGGAAGGGTCAAAT	50.0	50.0	57.9	57.8	413-416 bp	0.666667	71.3
ycf1_p2	TCCTCTGCCACGTTTTCTTC	CTGTCGTGGAAGGGTCAAAT	50.0	50.0	58.1	57.8	411-414 bp	0.666667	71.2
ycf1_p3	TATCAAAGCCACTTCGTCGG	CTGTCGTGGAAGGGTCAAAT	50.0	50.0	58.0	57.8	532-535 bp	0.666667	71.1
ycf1_p4	TCTCCCTCCTGTTGTTCCAA	CTGTCGTGGAAGGGTCAAAT	50.0	50.0	58.2	57.8	387-390 bp	0.666667	70.9
ycf1_p5	GATGATCCTCTGCCACGTTT	CTGTCGTGGAAGGGTCAAAT	50.0	50.0	58.0	57.8	419 bp	0.333333	51.6