Hotspot

rpl32-trnL-UAG

SSC · 117589-118320 · 732 bp

Back to Eugenia

Pi 0.0578

MarkerSeek score 77.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 732
variable_sites: 52
pi: 0.0578
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGTATATTGGGCAGCGTTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	657-868 bp	1.0	89.4
rpl32-trnL-UAG_p2	AGGTATATTGGGCAGCGTTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	658-869 bp	1.0	89.4
rpl32-trnL-UAG_p3	GGTATATTGGGCAGCGTTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	656-867 bp	1.0	89.4
rpl32-trnL-UAG_p4	GGTATATTGGGCAGCGTTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	657-868 bp	1.0	89.4
rpl32-trnL-UAG_p5	AGGTATATTGGGCAGCGTTG	ATCCCCATAAAAGAGCTGCG	50.0	50.0	58.0	58.0	1276-1492 bp	1.0	87.7