Hotspot

ndhF-rpl32

SSC · 116490-117414 · 925 bp

Back to Eugenia

Pi 0.0504

MarkerSeek score 79.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 925
variable_sites: 61
pi: 0.0504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGGGGAATTGTGCCGATTT	CAACGCTGCCCAATATACCT	45.0	50.0	58.1	58.0	1524-1761 bp	1.0	87.1
ndhF-rpl32_p2	AAAGGGGAATTGTGCCGATT	CAACGCTGCCCAATATACCT	45.0	50.0	58.1	58.0	1525-1762 bp	1.0	87.0
ndhF-rpl32_p3	TGCCGTTGGAAAAAGGAGAA	CAACGCTGCCCAATATACCT	45.0	50.0	57.9	58.0	1108 bp	0.333333	52.6
ndhF-rpl32_p4	TTGCCGTTGGAAAAAGGAGA	CAACGCTGCCCAATATACCT	45.0	50.0	57.9	58.0	1109 bp	0.333333	52.6
ndhF-rpl32_p5	TGCCGTTGGAAAAAGGAGAA	TCAACGCTGCCCAATATACC	45.0	50.0	57.9	58.0	1109 bp	0.333333	52.5