Hotspot

petA-psbJ

LSC · 66649-67665 · 1017 bp

Back to Eugenia

Pi 0.0416

MarkerSeek score 78.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1017
variable_sites: 61
pi: 0.0416
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATACTCCGGGTTGGGTTCAT	50.0	50.0	57.8	58.4	1133-1151 bp	1.0	83.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	TTCATACTCCGGGTTGGGTT	50.0	50.0	57.8	58.6	1136-1154 bp	1.0	81.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ATGGTTCATACTCCGGGTTG	50.0	50.0	57.8	57.3	1140-1158 bp	1.0	80.5
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	ATACTCCGGGTTGGGTTCA	50.0	52.6	57.8	57.9	1133-1151 bp	1.0	77.5
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	TACTCCGGGTTGGGTTCAT	50.0	52.6	57.8	57.9	1132-1150 bp	1.0	77.5