Hotspot

accD-psaI

LSC · 61961-62724 · 764 bp

Back to Eugenia

Pi 0.0433

MarkerSeek score 75.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 45
pi: 0.0433
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTGAGTTATTTAAGCTTCACGC	GGTAAGTTGTTAAGAGTTGTCA	39.1	36.4	57.5	54.0	688-829 bp	1.0	48.6
accD-psaI_p2	GTGAGTTATTTAAGCTTCACGC	GGTAAGTTGTTAAGAGTTGTCA	40.9	36.4	56.1	54.0	687-828 bp	1.0	48.6
accD-psaI_p3	AGTGAGTTATTTAAGCTTCACGC	GGTAAGTTGTTAAGAGTTGTCAT	39.1	34.8	57.5	54.6	688-829 bp	1.0	48.6
accD-psaI_p4	GTGAGTTATTTAAGCTTCACGC	GGTAAGTTGTTAAGAGTTGTCAT	40.9	34.8	56.1	54.6	687-828 bp	1.0	48.6
accD-psaI_p5	TGAGTTATTTAAGCTTCACGC	GGTAAGTTGTTAAGAGTTGTCA	38.1	36.4	54.6	54.0	686-827 bp	1.0	48.6