Hotspot

trnL-UAA-trnF-GAA

LSC · 51287-51460 · 174 bp

Back to Eugenia

Pi 0.0556
MarkerSeek score 79.30
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
174
variable_sites
14
pi
0.0556
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 220-230 bp 1.0 84.0
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 256-266 bp 1.0 82.5
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 232-242 bp 1.0 82.5
trnL-UAA-trnF-GAA_p4 TTCAAGTCCCTCTATCCCCA TCTACCAGCTGAGCTATCCC 50.0 55.0 57.0 58.0 209-219 bp 1.0 80.5
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 253-263 bp 1.0 79.3