Hotspot

trnfM-CAU-rps14

LSC · 40107-40256 · 150 bp

Back to Eugenia

Pi 0.0556

MarkerSeek score 78.80

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 150
variable_sites: 12
pi: 0.0556
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.0	58.1	219-229 bp	1.0	84.1
trnfM-CAU-rps14_p2	TTTGAACCCGTGACCTCAAG	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.0	58.1	236-246 bp	1.0	83.6
trnfM-CAU-rps14_p3	AAGGTTATGAGCCTTGCGAG	TTCGGGAAATGGTTCATGCA	50.0	45.0	58.0	58.1	249-259 bp	1.0	83.5
trnfM-CAU-rps14_p4	TTTGAACCCGTGACCTCAAG	TTCGGGAAATGGTTCATGCA	50.0	45.0	58.0	58.1	266-276 bp	1.0	83.1
trnfM-CAU-rps14_p5	AAGGTTATGAGCCTTGCGAG	GGCTTTCCGGACACATACTT	50.0	50.0	58.0	57.9	267-277 bp	1.0	83.0