Hotspot

rps2-rpoC2

LSC · 17694-17933 · 240 bp

Back to Eugenia

Pi 0.0505

MarkerSeek score 74.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 17
pi: 0.0505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ACCAAAATGAACTCCCGCTT	AGAATCGATCTGTTACCGCG	45.0	50.0	58.0	57.9	672-685 bp	1.0	83.7
rps2-rpoC2_p2	ACCTTTACGCTTGGCAGAAA	AGAATCGATCTGTTACCGCG	45.0	50.0	58.0	57.9	732-745 bp	1.0	83.3
rps2-rpoC2_p3	ACCAAAATGAACTCCCGCTT	TTGATTGGGGCTGAGCTAAC	45.0	50.0	58.0	57.9	899-912 bp	1.0	82.5
rps2-rpoC2_p4	GACAATTGAGTCTCCCCGTC	AGAATCGATCTGTTACCGCG	55.0	50.0	58.0	57.9	1006-1019 bp	1.0	82.3
rps2-rpoC2_p5	GACAATTGAGTCTCCCCGTC	TTGATTGGGGCTGAGCTAAC	55.0	50.0	58.0	57.9	1233-1246 bp	1.0	81.7