Hotspot

trnR-UCU-atpA

LSC · 10835-11189 · 355 bp

Back to Eugenia

Pi 0.0509

MarkerSeek score 76.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 23
pi: 0.0509
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CAGGAACAGAAGGAACGCTT	47.6	50.0	57.3	58.1	365-419 bp	1.0	74.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	398-452 bp	1.0	74.2
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCAGAAGC	47.6	50.0	57.3	57.8	404-458 bp	1.0	73.4
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	397-451 bp	1.0	71.9
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	396-450 bp	1.0	71.9