Hotspot

psbK-psbI

LSC · 8164-8549 · 386 bp

Back to Eugenia

Pi 0.0523

MarkerSeek score 77.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 28
pi: 0.0523
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	57.9	55.6	418-439 bp	1.0	53.7
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	415-436 bp	1.0	51.7
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	416-437 bp	1.0	51.7
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	417-438 bp	1.0	51.7
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACAAAGAGTTTGAGAGTAAGCA	50.0	34.8	57.9	56.3	419-440 bp	1.0	51.5