Hotspot

trnK-UUU-rps16

LSC · 4483-5054 · 572 bp

Back to Eugenia

Pi 0.0518

MarkerSeek score 80.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 43
pi: 0.0518
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	775-788 bp	1.0	87.6
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	913-926 bp	1.0	86.5
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	914-927 bp	1.0	86.5
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	AAGTGTATCGTGCGGGAATC	55.0	50.0	58.0	58.1	1433-1445 bp	1.0	86.0
trnK-UUU-rps16_p5	TTCATGGAAAAGCCCTTCCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	1065-1082 bp	1.0	85.9