Hotspot

trnH-GUG-psbA

LSC · 86-550 · 465 bp

Back to Eugenia

Pi 0.0871
MarkerSeek score 86.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
465
variable_sites
57
pi
0.0871
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 532-533 bp 1.0 90.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 546-547 bp 1.0 90.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 545-546 bp 1.0 90.4
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 535-536 bp 1.0 90.4
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 547-548 bp 1.0 90.3