Hotspot

rpl14

LSC · 81109-81477 · 369 bp

Back to Euchiton

Pi 0.00542

MarkerSeek score 42.70

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 2
pi: 0.00542
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	ACTCGAACCACGCTTTTTCT	CCAGTTACGGTAAGACCTGC	45.0	55.0	58.1	58.0	938-946 bp	1.0	80.1
rpl14_p2	AGAATTACAACGCCCATCCC	CCAGTTACGGTAAGACCTGC	50.0	55.0	57.9	58.0	1184-1192 bp	1.0	80.0
rpl14_p3	ACTCGAACCACGCTTTTTCT	ATACGCTCTTCAGGCACTTG	45.0	50.0	58.1	58.0	1062-1070 bp	1.0	80.0
rpl14_p4	AGAATTACAACGCCCATCCC	ATACGCTCTTCAGGCACTTG	50.0	50.0	57.9	58.0	1308-1316 bp	1.0	79.9
rpl14_p5	TTTCGATAAAACCCTCGCGT	CCAGTTACGGTAAGACCTGC	45.0	55.0	57.9	58.0	1000-1008 bp	1.0	79.8