Hotspot

psbA

LSC · 323-1402 · 1080 bp

Back to Euchiton

Pi 0

MarkerSeek score 28.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1080
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCCTATACAATTAGACCAAAGTC	AAATTCGTGTGCTTGGGAGT	36.0	45.0	56.1	58.0	1138 bp	1.0	40.0
psbA_p2	ACTCCTATACAATTAGACCAAAGTC	TGCTTGGGAGTCCCTGATAA	36.0	50.0	56.1	58.0	1129 bp	1.0	40.0
psbA_p3	ACTCCTATACAATTAGACCAAAGT	AAATTCGTGTGCTTGGGAGT	33.3	45.0	55.0	58.0	1138 bp	1.0	40.0
psbA_p4	ACTCCTATACAATTAGACCAAAGT	TGCTTGGGAGTCCCTGATAA	33.3	50.0	55.0	58.0	1129 bp	1.0	40.0
psbA_p5	AACTCCTATACAATTAGACCAAAGTC	AAATTCGTGTGCTTGGGAGT	34.6	45.0	56.8	58.0	1139 bp	1.0	40.0