Hotspot

ndhF-ycf1

SSC · 127682-127718 · 37 bp

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Pi 0.0417

MarkerSeek score 71.00

Primer available yes

Label ndhF-ycf1

Feature Metadata

label_name: ndhF-ycf1
feature_type: igs
region: SSC
length_bp: 37
variable_sites: 1
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-ycf1_p1	GGGTGGGCGTATTTCTTCTT	TGTTTCGATGCAACAGCAAG	50.0	45.0	57.9	57.6	209-222 bp	1.0	80.1
ndhF-ycf1_p2	GGGTGGGCGTATTTCTTCTT	GTTTCGATGCAACAGCAAGA	50.0	45.0	57.9	57.3	208-221 bp	1.0	77.9
ndhF-ycf1_p3	GGGTGGGCGTATTTCTTCTT	TTCGATGCAACAGCAAGATG	50.0	45.0	57.9	57.1	206-219 bp	1.0	76.1
ndhF-ycf1_p4	GGGTGGGCGTATTTCTTCTT	CGATGCAACAGCAAGATGC	50.0	52.6	57.9	58.1	204-217 bp	1.0	74.9
ndhF-ycf1_p5	GGGTGGGCGTATTTCTTCTT	ATGTTTCGATGCAACAGCAAG	50.0	42.9	57.9	58.1	210-223 bp	1.0	74.9