Hotspot

psbB-psbT

LSC · 74385-74579 · 195 bp

Back to Euchiton

Pi 0

MarkerSeek score 20.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TTCTTCGGACACATTTGGCA	ACCGCAAATAAAGCCATTGC	45.0	45.0	58.0	58.0	913-914 bp	1.0	80.2
psbB-psbT_p2	GGGGATGGAATAGCAGTTGG	ACCGCAAATAAAGCCATTGC	55.0	45.0	58.0	58.0	1303-1304 bp	1.0	80.1
psbB-psbT_p3	GGCCGTGAACTTTTTGTACG	ACCGCAAATAAAGCCATTGC	50.0	45.0	58.0	58.0	1249-1250 bp	1.0	80.0
psbB-psbT_p4	TGTACGTCGTATGCCTACCT	ACCGCAAATAAAGCCATTGC	50.0	45.0	58.0	58.0	1235-1236 bp	1.0	79.9
psbB-psbT_p5	GTTCCTTTTCGAAGGGCAGA	ACCGCAAATAAAGCCATTGC	50.0	45.0	58.1	58.0	1159-1160 bp	1.0	79.4