Hotspot

accD-psaI

LSC · 58865-59634 · 770 bp

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Pi 0.0104

MarkerSeek score 54.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 8
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	ACCTCGATTTACTATTTGTACCTGT	47.6	36.0	57.2	57.8	908-912 bp	1.0	40.8
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	ACCTCGATTTACTATTTGTACCTGT	47.6	36.0	57.2	57.8	907-911 bp	1.0	40.8
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	ACCTCGATTTACTATTTGTACCTGT	45.0	36.0	56.2	57.8	909-913 bp	1.0	40.8
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	ACCTCGATTTACTATTTGTACCTGT	45.0	36.0	56.0	57.8	908-912 bp	1.0	40.8
accD-psaI_p5	TTCCAGAAGGGCTTATTCGATC	ACCTCGATTTACTATTTGTACCTGT	45.5	36.0	57.9	57.8	909-913 bp	1.0	40.8