Hotspot

trnT-UGU-trnL-UAA

LSC · 46676-47238 · 563 bp

Back to Euchiton

Pi 0.00535

MarkerSeek score 61.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 3
pi: 0.00535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	622-624 bp	1.0	77.6
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	654-656 bp	1.0	76.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	616-618 bp	1.0	76.3
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	648-650 bp	1.0	75.4
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	621-623 bp	1.0	74.6