Hotspot

trnE-UUC-rpoB

LSC · 11830-12736 · 907 bp

Back to Euchiton

Pi 0

MarkerSeek score 61.80

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGGTATTGTGGACATTGCGT	50.0	45.0	58.0	57.5	964-993 bp	1.0	78.3
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	AGGTATTGTGGACATTGCGT	55.0	45.0	58.1	57.5	923-952 bp	1.0	77.7
trnE-UUC-rpoB_p3	TGATAGGAAATCCCGCTCCT	AGGTATTGTGGACATTGCGT	50.0	45.0	57.9	57.5	1012-1041 bp	1.0	77.5
trnE-UUC-rpoB_p4	CCCTCGTTTTCTCCGTGAAA	AGGTATTGTGGACATTGCGT	50.0	45.0	58.1	57.5	954-983 bp	1.0	77.5
trnE-UUC-rpoB_p5	TTGAACCCTCGTTTTCTCCG	AGGTATTGTGGACATTGCGT	50.0	45.0	58.1	57.5	959-988 bp	1.0	77.5