Hotspot

trnC-GCA-petN

LSC · 9379-9967 · 589 bp

Back to Euchiton

Pi 0.00513

MarkerSeek score 54.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 3
pi: 0.00513
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	641-645 bp	1.0	79.0
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	659-663 bp	1.0	76.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	640-644 bp	1.0	73.1
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	649-653 bp	1.0	73.0
trnC-GCA-petN_p5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	698-702 bp	1.0	72.3