Hotspot

psbI-trnS-GCU

LSC · 8310-8451 · 142 bp

Back to Euchiton

Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 2
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	257 bp	1.0	72.3
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	TTTCGATTGGGAGAGATGGC	50.0	50.0	57.1	57.7	262 bp	1.0	70.5
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	205 bp	1.0	70.1
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	257 bp	1.0	69.9
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	TTCGATTGGGAGAGATGGCT	50.0	50.0	57.1	58.5	261 bp	1.0	69.1