Hotspot

trnK-UUU-rps16

LSC · 4230-5046 · 817 bp

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Pi 0.00629
MarkerSeek score 50.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
817
variable_sites
5
pi
0.00629
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1225-1243 bp 1.0 81.1
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1224-1242 bp 1.0 81.1
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG AAACGTTCCCGCAATTCTCT 55.0 45.0 58.0 58.1 1051-1070 bp 1.0 81.1
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG CCCAGAACAAGGAAACACCA 55.0 50.0 58.0 57.9 1532-1550 bp 1.0 80.9
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG CCCAGAACAAGGAAACACCA 55.0 50.0 58.0 57.9 1531-1549 bp 1.0 80.9