Hotspot

rps19-rpl2

IRb · 88905-88965 · 61 bp

Back to Eucalyptus

Pi 0.085

MarkerSeek score 74.50

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 61
variable_sites: 16
pi: 0.085
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCCGCCTTTGTGTTAAGCTT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	156-174 bp	1.0	91.0
rps19-rpl2_p2	TTCCGCCTTTGTGTTAAGCT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	157-175 bp	1.0	91.0
rps19-rpl2_p3	TCCGCCTTTGTGTTAAGCTT	ATTTGATTCTTCGTCGCCGT	45.0	45.0	58.0	58.3	158-176 bp	1.0	89.6
rps19-rpl2_p4	TCCGCCTTTGTGTTAAGCTT	TTGGGGTTATCCTGCACTTG	45.0	50.0	58.0	57.8	214-232 bp	1.0	89.4
rps19-rpl2_p5	TTCCGCCTTTGTGTTAAGCT	TTGGGGTTATCCTGCACTTG	45.0	50.0	58.0	57.8	215-233 bp	1.0	89.3