Hotspot

petA-psbJ

LSC · 67354-68348 · 995 bp

Back to Eucalyptus

Pi 0.0122

MarkerSeek score 57.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 65
pi: 0.0122
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTGGGTGGATTTGGTCAAG	TGGACTAGCTGTACCTACCG	50.0	55.0	58.0	58.0	1613-1647 bp	1.0	84.5
petA-psbJ_p2	TGTGGGTGGATTTGGTCAAG	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1612-1646 bp	1.0	84.5
petA-psbJ_p3	TGTGGGTGGATTTGGTCAAG	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1754-1788 bp	1.0	84.2
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1824-1858 bp	1.0	84.0
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	2144-2178 bp	1.0	83.5