Hotspot

accD-psaI

LSC · 62647-63405 · 759 bp

Back to Eucalyptus

Pi 0.00921

MarkerSeek score 70.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 56
pi: 0.00921
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAATCGTACCACGTAATCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	897-929 bp	1.0	76.2
accD-psaI_p2	CCAATCGTACCACGTAATCCT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	896-928 bp	1.0	76.2
accD-psaI_p3	TCCAATCGTACCACGTAATCC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.6	57.9	903-935 bp	1.0	75.6
accD-psaI_p4	CCAATCGTACCACGTAATCCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.6	57.9	902-934 bp	1.0	75.5
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	872-904 bp	1.0	74.3