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EARO_CP_t001-psbA

LSC · 77-556 · 480 bp

Back to Eucalyptus

Pi 0.022

MarkerSeek score 71.30

Primer available yes

Label EARO_CP_t001-psbA

Feature Metadata

label_name: EARO_CP_t001-psbA
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 64
pi: 0.022
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EARO_CP_t001-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	383-583 bp	1.0	90.4
EARO_CP_t001-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	397-597 bp	1.0	90.4
EARO_CP_t001-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	396-596 bp	1.0	90.4
EARO_CP_t001-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	398-598 bp	1.0	90.4
EARO_CP_t001-psbA_p5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	386-586 bp	1.0	90.4