Hotspot

ndhF-rpl32

SSC · 111479-112516 · 1038 bp

Back to Eschenbachia

Pi 0.0205

MarkerSeek score 64.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1038
variable_sites: 21
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACGACGCCCAATATCCTTTC	50.0	50.0	58.0	58.1	1221-1226 bp	1.0	81.6
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	ACGACGCCCAATATCCTTTC	45.0	50.0	58.0	58.1	1219-1224 bp	1.0	81.5
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	ACGACGCCCAATATCCTTTC	45.0	50.0	58.0	58.1	1220-1225 bp	1.0	81.5
ndhF-rpl32_p4	CTTTTTGTTGCCGTCGGAAA	ACGACGCCCAATATCCTTTC	45.0	50.0	57.8	58.1	1227-1232 bp	1.0	80.3
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	ACGACGCCCAATATCCTTTC	45.0	50.0	57.8	58.1	1224-1229 bp	1.0	80.3