Hotspot

ycf1-ndhF

SSC · 109235-109252 · 18 bp

Back to Eschenbachia

Pi 0.0556

MarkerSeek score 68.80

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TTCTTCCTTCTCCGAGCTCA	TGTGGGGACGAGAAAATTGG	50.0	50.0	58.1	58.1	670 bp	1.0	79.5
ycf1-ndhF_p2	CTAGCACTGGGTAGACCTCA	TGTGGGGACGAGAAAATTGG	55.0	50.0	57.9	58.1	541 bp	1.0	79.0
ycf1-ndhF_p3	TCGTTGTGGTCGGACTCTAT	TGTGGGGACGAGAAAATTGG	50.0	50.0	58.2	58.1	729 bp	1.0	78.6
ycf1-ndhF_p4	ATCAATTCGGTCGTTGTGGT	TGTGGGGACGAGAAAATTGG	45.0	50.0	57.8	58.1	739 bp	1.0	78.6
ycf1-ndhF_p5	GGGTATCAGCAACAACTGGT	TGTGGGGACGAGAAAATTGG	50.0	50.0	57.8	58.1	615 bp	1.0	78.4