Hotspot

rpl33-rps18

LSC · 67915-68099 · 185 bp

Back to Eschenbachia

Pi 0.017

MarkerSeek score 66.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 3
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGCACAATACGCCTAATCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	348-357 bp	1.0	79.6
rpl33-rps18_p2	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	45.0	50.0	58.2	57.7	361-370 bp	1.0	78.7
rpl33-rps18_p3	GGCACAATACGCCTAATCGA	ATTCGATCCCCCGATTGGAT	50.0	50.0	58.1	58.6	345-354 bp	1.0	76.5
rpl33-rps18_p4	TACTCAAAAGAACCGGCACA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.4	57.7	362-371 bp	1.0	75.6
rpl33-rps18_p5	ACTCAAAAGAACCGGCACAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	58.2	58.6	358-367 bp	1.0	75.6