Hotspot

trnS-GGA-rps4

LSC · 45295-45592 · 298 bp

Back to Eschenbachia

Pi 0.0369
MarkerSeek score 66.70
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
298
variable_sites
11
pi
0.0369
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 ATGTAGGAGAGATGGCCGAG AGTAAATGGGTCGGTTTGAA 55.0 40.0 58.4 54.9 455-460 bp 1.0 53.9
trnS-GGA-rps4_p2 TAGGCTTTTGCTTACCGAGG AGTAAATGGGTCGGTTTGAA 50.0 40.0 57.6 54.9 404-409 bp 1.0 53.9
trnS-GGA-rps4_p3 TTTTGCTTACCGAGGGTTCG AGTAAATGGGTCGGTTTGAA 50.0 40.0 58.5 54.9 399-404 bp 1.0 53.3
trnS-GGA-rps4_p4 TAGGCTTTTGCTTACCGAGG AGTAAATGGGTCGGTTTGAAA 50.0 38.1 57.6 55.7 404-409 bp 1.0 52.6
trnS-GGA-rps4_p5 ATGTAGGAGAGATGGCCGAG AGTAAATGGGTCGGTTTGAAA 55.0 38.1 58.4 55.7 455-460 bp 1.0 52.5