Hotspot

ycf3-trnS-GGA

LSC · 44349-45208 · 860 bp

Back to Eschenbachia

Pi 0.0176
MarkerSeek score 65.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
860
variable_sites
15
pi
0.0176
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1025-1032 bp 1.0 67.3
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1026-1033 bp 1.0 67.3
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA CCTCGGTAAGCAAAAGCCTA 45.0 50.0 56.4 57.6 1015-1022 bp 1.0 65.9
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA CCTCGGTAAGCAAAAGCCTA 45.0 50.0 56.4 57.6 1016-1023 bp 1.0 65.9
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA GATTCGAACCCTCGGTAAGC 45.0 55.0 56.4 58.4 1024-1031 bp 1.0 65.6