Hotspot

rps14-psaB

LSC · 37017-37149 · 133 bp

Back to Eschenbachia

Pi 0.0376

MarkerSeek score 72.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 5
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGCCTCTTCTTTTCCCTCTG	GCAGCTTTCTTGATTGCCTC	50.0	50.0	57.1	57.7	216-217 bp	1.0	72.7
rps14-psaB_p2	TGCCTCTTCTTTTCCCTCTG	TGCAGCTTTCTTGATTGCCT	50.0	45.0	57.1	58.4	217-218 bp	1.0	71.9
rps14-psaB_p3	TGCCTCTTCTTTTCCCTCTG	CGGTGGCACTTTCCATTGTA	50.0	50.0	57.1	58.5	290-291 bp	1.0	70.7
rps14-psaB_p4	TGTCACTCAACGATCGAACT	GCAGCTTTCTTGATTGCCTC	45.0	50.0	56.9	57.7	293-294 bp	1.0	70.5
rps14-psaB_p5	TTGCCTCTTCTTTTCCCTCTG	GCAGCTTTCTTGATTGCCTC	47.6	50.0	57.9	57.7	217-218 bp	1.0	70.5