Hotspot

trnT-GGU-psbD

LSC · 31316-32637 · 1322 bp

Back to Eschenbachia

Pi 0.0123

MarkerSeek score 57.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1322
variable_sites: 16
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTAAGCCCTCGGTTCAGATC	GAAACGGTCCCTACGTAACC	55.0	55.0	57.8	58.0	1438-1450 bp	1.0	80.4
trnT-GGU-psbD_p2	GTAAGCCCTCGGTTCAGATC	GGACCAGCCTACAAAAACGA	55.0	50.0	57.8	58.1	1456-1468 bp	1.0	79.5
trnT-GGU-psbD_p3	GTAAGCCCTCGGTTCAGATC	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.8	58.2	1446-1458 bp	1.0	78.4
trnT-GGU-psbD_p4	GTAAGCCCTCGGTTCAGATC	CGAAACGGTCCCTACGTAAC	55.0	55.0	57.8	58.4	1439-1451 bp	1.0	77.3
trnT-GGU-psbD_p5	GTAATCCCATGGTAAGGCGT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.4	58.0	1456-1468 bp	1.0	77.1