Hotspot

atpI-atpH

LSC · 24963-26114 · 1152 bp

Back to Eschenbachia

Pi 0.0106

MarkerSeek score 66.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1152
variable_sites: 12
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	AGTGGTTCCGATCCCTGTTA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.1	58.0	1288-1299 bp	1.0	81.0
atpI-atpH_p2	AGTGGTTCCGATCCCTGTTA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.1	57.9	1344-1355 bp	1.0	80.3
atpI-atpH_p3	AGTGGTTCCGATCCCTGTTA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.1	57.9	1304-1315 bp	1.0	80.1
atpI-atpH_p4	AGTGGTTCCGATCCCTGTTA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.1	58.2	1289-1300 bp	1.0	79.8
atpI-atpH_p5	AGTGGTTCCGATCCCTGTTA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.1	58.2	1348-1359 bp	1.0	79.7