Hotspot

ndhF-rpl32

SSC · 114258-115180 · 923 bp

Back to Erythrostemon

Pi 0.0328
MarkerSeek score 68.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
923
variable_sites
29
pi
0.0328
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAATATTGATGTGTATGTTCCA GCTGCCCAATATCCCTTCTC 31.8 55.0 52.0 58.1 999-1029 bp 1.0 45.4
ndhF-rpl32_p2 GAATATTGATGTGTATGTTCCA TGCCCAATATCCCTTCTCCT 31.8 50.0 52.0 57.8 997-1027 bp 1.0 45.4
ndhF-rpl32_p3 GAATATTGATGTGTATGTTCCA TCAATGCTGCCCAATATCCC 31.8 50.0 52.0 58.0 1004-1034 bp 1.0 45.3
ndhF-rpl32_p4 GAATATTGATGTGTATGTTCCA CAATGCTGCCCAATATCCCT 31.8 50.0 52.0 58.0 1003-1033 bp 1.0 45.3
ndhF-rpl32_p5 GAATATTGATGTGTATGTTCCA AAAAGCTTTCAATGCTGCCC 31.8 45.0 52.0 57.8 1012-1042 bp 1.0 45.3