Hotspot

rps3-rps19

LSC · 85125-85779 · 655 bp

Back to Erythrostemon

Pi 0.0642

MarkerSeek score 89.50

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 41
pi: 0.0642
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	TTAAACTTCCGGGGACATGC	50.0	50.0	57.5	58.2	739-743 bp	1.0	81.2
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	TTAAACTTCCGGGGACATGC	50.0	50.0	58.6	58.2	733-737 bp	1.0	80.4
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	TTAAACTTCCGGGGACATGC	45.0	50.0	57.0	58.2	777-781 bp	1.0	76.8
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	TTAAACTTCCGGGGACATGC	45.0	50.0	57.0	58.2	732-736 bp	1.0	76.5
rps3-rps19_p5	GTCTGAAACCGAGCGGATT	TTAAACTTCCGGGGACATGC	52.6	50.0	57.9	58.2	733-737 bp	1.0	75.7