Hotspot

rpl36-rps8

LSC · 81252-81726 · 475 bp

Back to Erythrostemon

Pi 0.0422

MarkerSeek score 67.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 20
pi: 0.0422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TACGCGCATTCTTACGTGAA	GTTTCATTAGCACCCGAGGT	45.0	50.0	58.0	57.9	1526-1602 bp	1.0	82.6
rpl36-rps8_p2	TTACGCGCATTCTTACGTGA	GTTTCATTAGCACCCGAGGT	45.0	50.0	58.0	57.9	1527-1603 bp	1.0	82.6
rpl36-rps8_p3	GAAATCACCTGACCCCGTAC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.0	57.9	1611-1687 bp	1.0	82.5
rpl36-rps8_p4	TTTTAGGGGGCCTACATCCA	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.0	57.9	1859-1935 bp	1.0	82.1
rpl36-rps8_p5	ATAATTCGTCCGCGTCTACG	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.0	57.9	1193-1269 bp	1.0	81.5