Hotspot

rps11-rpl36

LSC · 80918-81137 · 220 bp

Back to Erythrostemon

Pi 0.0735

MarkerSeek score 76.00

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 15
pi: 0.0735
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTCTACGTGAACCA	CGTAGACGCGGACGAATTAT	47.6	50.0	58.3	58.0	327 bp	1.0	76.8
rps11-rpl36_p2	CGTCCATTTCTACGTGAACC	CGTAGACGCGGACGAATTAT	50.0	50.0	56.6	58.0	327 bp	1.0	75.9
rps11-rpl36_p3	CGTCCATTTCTACGTGAACCA	TGTCGACTGATCCGTAGACG	47.6	55.0	58.3	59.0	339 bp	1.0	68.8
rps11-rpl36_p4	CGTCCATTTCTACGTGAACC	TGTCGACTGATCCGTAGACG	50.0	55.0	56.6	59.0	339 bp	1.0	67.9
rps11-rpl36_p5	CGTCCATTTCTACGTGAACCA	CGTAGACGCGGACGAATTATA	47.6	47.6	58.3	57.8	327 bp	1.0	67.6