Hotspot

rpl33-rps18

LSC · 69294-69496 · 203 bp

Back to Erythrostemon

Pi 0.0985

MarkerSeek score 81.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 20
pi: 0.0985
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	339 bp	1.0	81.6
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	340 bp	1.0	81.6
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	341 bp	1.0	79.7
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	336 bp	1.0	78.6
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	337 bp	1.0	78.6