Hotspot

accD-psaI

LSC · 60495-60770 · 276 bp

Back to Erythrostemon

Pi 0.0698

MarkerSeek score 67.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 276
variable_sites: 15
pi: 0.0698
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTACTACTGGTGGGGTGACA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	574-615 bp	1.0	85.9
accD-psaI_p2	TACTACTGGTGGGGTGACAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	573-614 bp	1.0	85.9
accD-psaI_p3	GTATGTGCTTCTGGAGGAGC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	704-745 bp	1.0	85.7
accD-psaI_p4	TGGTCTCTGTGGATCCCATT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	993 bp	0.5	59.6
accD-psaI_p5	TGGTCTCTGTGGATCCCATT	AAAAGAATTTGCTGCGACGG	50.0	45.0	58.0	57.9	1401 bp	0.5	59.4