Hotspot

rps4-trnT-UGU

LSC · 47347-47695 · 349 bp

Back to Erythrostemon

Pi 0.0511

MarkerSeek score 74.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 17
pi: 0.0511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	427-439 bp	1.0	82.8
rps4-trnT-UGU_p2	GAAACGAGGCCCTCTGTAAC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	395-407 bp	1.0	82.4
rps4-trnT-UGU_p3	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	432-444 bp	1.0	82.1
rps4-trnT-UGU_p4	TTTTGAAACGAGGCCCTCTG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	431-443 bp	1.0	81.8
rps4-trnT-UGU_p5	GAAACGAGGCCCTCTGTAAC	TGTTATGTGAGCCCGCTTAG	55.0	50.0	58.3	57.7	447-459 bp	1.0	81.1