Hotspot

trnG-GCC-trnfM-CAU

LSC · 38045-38235 · 191 bp

Back to Erythrostemon

Pi 0.0492

MarkerSeek score 76.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 9
pi: 0.0492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CTTTGCCAAAGAGAAGACGC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.7	58.0	260-268 bp	1.0	82.3
trnG-GCC-trnfM-CAU_p2	CTTTGCCAAAGAGAAGACGC	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.7	57.8	258-266 bp	1.0	81.2
trnG-GCC-trnfM-CAU_p3	CTTTGCCAAAGAGAAGACGC	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.7	57.8	281-289 bp	1.0	80.4
trnG-GCC-trnfM-CAU_p4	CTTTGCCAAAGAGAAGACGC	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.7	57.8	280-288 bp	1.0	80.4
trnG-GCC-trnfM-CAU_p5	CTTTGCCAAAGAGAAGACGC	ATTTTCTCTTTACCGCGGGG	50.0	50.0	57.7	58.3	313-321 bp	1.0	79.7