Hotspot

atpF-atpH

LSC · 13324-13794 · 471 bp

Back to Erythrostemon

Pi 0.0495

MarkerSeek score 81.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 21
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAATCCGAAACTCCCAGCG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	58.0	57.6	556-587 bp	1.0	83.4
atpF-atpH_p2	TAAATCCGAAACTCCCAGCG	AGGCAGAGGGAAAAATACGG	50.0	50.0	58.0	57.6	623-654 bp	1.0	83.1
atpF-atpH_p3	TAAATCCGAAACTCCCAGCG	GCAGAGGGAAAAATACGGGG	50.0	55.0	58.0	58.6	621-652 bp	1.0	81.6
atpF-atpH_p4	TAAATCCGAAACTCCCAGCG	GGCAGAGGGAAAAATACGGG	50.0	55.0	58.0	58.6	622-653 bp	1.0	81.6
atpF-atpH_p5	TAAATCCGAAACTCCCAGCG	CAGAGGGAAAAATACGGGGT	50.0	50.0	58.0	57.2	620-651 bp	1.0	80.1