Hotspot

trnG-UCC-trnR-UCU

LSC · 9665-10032 · 368 bp

Back to Erythrostemon

Pi 0.0424

MarkerSeek score 75.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 14
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	420-448 bp	1.0	69.9
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	423-451 bp	1.0	69.7
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	426-454 bp	1.0	69.2
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	429-457 bp	1.0	69.0
trnG-UCC-trnR-UCU_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	490-518 bp	1.0	67.7