Hotspot

psbI-trnS-GCU

LSC · 8097-8171 · 75 bp

Back to Erythrostemon

Pi 0.0933

MarkerSeek score 80.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 7
pi: 0.0933
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CCTATCTAACGATCCAGGACG	TGGACTAAAGCGTCGGATTG	52.4	50.0	57.2	58.0	191-310 bp	1.0	67.5
psbI-trnS-GCU_p2	CCTATCTAACGATCCAGGACG	GTGGACTAAAGCGTCGGATT	52.4	50.0	57.2	58.0	192-311 bp	1.0	67.5
psbI-trnS-GCU_p3	CCTATCTAACGATCCAGGACG	TTTTTCGTACCGAGGGTTCG	52.4	50.0	57.2	58.2	154-273 bp	1.0	66.2
psbI-trnS-GCU_p4	CCTATCTAACGATCCAGGACG	CAATTGGGGAGAGATGGCTG	52.4	55.0	57.2	58.3	213-332 bp	1.0	64.9
psbI-trnS-GCU_p5	CCTATCTAACGATCCAGGACG	GACTAAAGCGTCGGATTGCT	52.4	50.0	57.2	58.4	189-308 bp	1.0	64.7